Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2

• Published in: Virology journal Vol. 7; no. 1; p. 274
• Main Authors: Sun, Shi-Qi, Guo, Hui-Chen, Sun, De-Hui, Yin, Shuang-Hui, Shang, You-Jun, Cai, Xue-Peng, Liu, Xiang-Tao
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19-10-2010; BioMed Central; BMC
• Subjects: Animals; Antibodies, Viral - blood; Antigens, Viral; Capsid Proteins; Circoviridae Infections - diagnosis; Circoviridae Infections - veterinary; Circovirus - immunology; Circovirus - isolation & purification; Cloning; Deoxyribonucleic acid; Diagnosis; DNA; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Escherichia coli; Escherichia coli - genetics; Genetic aspects; Genomes; Hogs; Milk; Physiological aspects; Porcine circovirus; Recombinant Proteins; Sensitivity and Specificity; Standard deviation; Swine; Swine Diseases - diagnosis; Swine Diseases - virology; Technological change; Viral proteins; Virology - methods; Virus diseases; China
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-7-274

The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain (113-147AA) of ORF2-encoded antigen, expressed in E. coli, for diagnosis of PCV infection.
The ELISA was performed on 288 serum samples collected from different porcine herds and compared with an indirect immunofluorescent assay (IFA). In total, 262 of 288 samples were positive as indicated by both I-ELISA and IFA. The specificity and sensitivity of I-ELISA were 87.7% and 93.57%.
This ELISA is suitable for detection and discrimination of PCV2 infection in both SPF and farm antisera.