Combined histomorphometric and gene-expression profiling applied to toxicology

• Published in: Genome biology Vol. 4; no. 5; p. R32
• Main Authors: Kriete, Andres, Anderson, Mary K, Love, Brad, Freund, John, Caffrey, James J, Young, M Brook, Sendera, Timothy J, Magnuson, Scott R, Braughler, J Mark
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01-01-2003; BioMed Central
• Subjects: Animals; Carbon Tetrachloride - toxicity; Chemical and Drug Induced Liver Injury; Data Interpretation, Statistical; Gene Expression Profiling; Histocytochemistry - methods; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver Diseases - genetics; Liver Diseases - pathology; Method; Oligonucleotide Array Sequence Analysis - methods; Rats; Rats, Sprague-Dawley; RNA - genetics; RNA - metabolism; Toxicology - methods
• ISSN: 1474-760X; 1465-6906; 1474-760X; 1465-6914
• DOI: 10.1186/gb-2003-4-5-r32

We have developed a unique methodology for the combined analysis of histomorphometric and gene-expression profiles amenable to intensive data mining and multisample comparison for a comprehensive approach to toxicology. This hybrid technology, termed extensible morphometric relational gene-expression analysis (EMeRGE), is applied in a toxicological study of time-varied vehicle- and carbon-tetrachloride (CCl4)-treated rats, and demonstrates correlations between specific genes and tissue structures that can augment interpretation of biological observations and diagnosis.