Measurement of Tumor Oxygenation: In Vivo Comparison of a Luminescence Fiber-optic Sensor and a Polarographic Electrode in the P22 Tumor

• Published in: Radiation research Vol. 155; no. 6; pp. 837 - 846
• Main Authors: Seddon, Beatrice M., Honess, Davina J., Vojnovic, Borivoj, Tozer, Gillian M., Workman, Paul
• Format: Journal Article
• Language: English
• Published: Oak Brook, Il Radiation Research Society 01-06-2001
• Subjects: Animals; Biological and medical sciences; Biosensing Techniques; Cancer; Carbon monoxide; Carbon Monoxide - metabolism; Carcinosarcoma - metabolism; Electrodes; Fiber Optic Technology; General aspects (metabolism, cell proliferation, established cell line...); Hypoxia; Luminescent Measurements; Medical sciences; Nadir; Neoplasms, Experimental - metabolism; Optical Fibers; Oxygen; Oxygen - metabolism; Polarography; REGULAR ARTICLES; Sensors; Space life sciences; Statistical median; Tissue oxygenation; Tumor cell; Tumors; Biomedical equipment; Rat; Rodentia; Luminescence; Exploration; Vertebrata; Cancerology; Mammalia; Polarography; Animal; Tumor; Oxygenation; Tumor cell; Comparative study
• ISSN: 0033-7587; 1938-5404
• DOI: 10.1667/0033-7587(2001)155[0837:MOTOIV]2.0.CO;2

Seddon, B. M., Honess, D. J., Vojnovic, B., Tozer, G. M. and Workman, P. Measurement of Tumor Oxygenation: In Vivo Comparison of a Luminescence Fiber-optic Sensor and a Polarographic Electrode in the P22 Tumor. Radiat. Res. 155, 835–844 (2001). Hypoxia is important in tumor biology and therapy. This study compared the novel luminescence fiber-optic OxyLite sensor with the Eppendorf polarographic electrode in measuring tumor oxygenation. Using the relatively well-oxygenated P22 tumor, oxygen measurements were made with both instruments in the same individual tumors. In 24 air-breathing animals, pooled electrode pO2 readings lay in a range over twice that of sensor pO2(5min) values (–3.2 to 80 mmHg and –0.1 to 34.8 mmHg, respectively). However, there was no significant difference between the means ± 2 SE of the median pO2 values recorded by each instrument (11.0 ± 3.3 and 8.1 ± 1.9 mmHg, for the electrode and sensor respectively, P = 0.07). In a group of 12 animals treated with carbon monoxide inhalation to induce tumor hypoxia, there was a small but significant difference between the means ± 2 SE of the median pO2 values reported by the electrode and sensor (1.7 ± 0.9 and 2.9 ± 0.7 mmHg, respectively, P = 0.009). A variable degree of disparity was seen on comparison of pairs of median pO2 values from individual tumors in both air-breathing and carbon monoxide-breathing animals. Despite the differences between the sets of readings made with each instrument from individual tumors, we have shown that the two instruments provide comparable assessments of tumor oxygenation in groups of tumors, over the range of median pO2 values of 0.6 to 28.1 mmHg.