Cell cycle related proteins as prognostic parameters in radically resected non-small cell lung cancer

• Published in: Journal of clinical pathology Vol. 58; no. 7; pp. 734 - 739
• Main Authors: Esposito, V, Baldi, A, De Luca, A, Tonini, G, Vincenzi, B, Santini, D, Persichetti, P, Mancini, A, Citro, G, Baldi, F, Groeger, A M, Caputi, M
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01-07-2005; BMJ; BMJ Publishing Group LTD; Copyright 2005 Journal of Clinical Pathology
• Subjects: Aged; Aged, 80 and over; Biological and medical sciences; Biomarkers, Tumor - metabolism; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Non-Small-Cell Lung - surgery; Cell Cycle Proteins - metabolism; confidence interval; cyclin D1; Cyclin D1 - metabolism; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; Female; Humans; Immunoenzyme Techniques; immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Lung cancer; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Lung Neoplasms - surgery; Male; Medical sciences; Middle Aged; Neoplasm Proteins - metabolism; Neoplasm Staging; non-small cell lung cancer; NSCLC; Original; p16; p53; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; PCNA; Pneumology; pRb; pRb2/p130; Prognosis; proliferating nuclear cell antigen; Proteins - metabolism; retinoblastoma protein; Retinoblastoma-Like Protein p130; Survival Analysis; Tumors of the respiratory system and mediastinum; Lung disease; Anatomic pathology; Prognosis; Respiratory disease; Lung cancer; Cell cycle; Bronchus disease; Parameter; Malignant tumor; non-small cell lung carcinoma; Protein
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.2004.023531

Background: Experimental evidence suggests that lung cancer development and progression can be linked to an increased proliferation rate. Aims/Methods: To evaluate the immunohistochemical expression of seven components of the cell cycle machinery in a series of well characterised non-small cell lung cancer (NSCLC) specimens (n  =  105). Results: Multivariate analysis revealed that simultaneous loss of expression of three of these factors—cyclin D1, the cyclin dependent kinase inhibitor p16, and the tumour suppressor retinoblastoma protein Rb2/p130—correlated with survival, confirming the hypothesis that the cyclin D1–p16–retinoblastoma tumour suppressor pathway is inactivated in most lung cancer samples. Conclusions: These results suggest that loss of control of cell cycle checkpoints is a common occurrence in lung cancer and support the idea that functional cooperation between different cell cycle regulatory proteins constitutes another level of regulation in cell growth control and tumour suppression.