High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus

• Published in: Arthritis research & therapy Vol. 12; no. 1; p. R6
• Main Authors: Vázquez-Del Mercado, Monica, Palafox-Sánchez, Claudia A, Muñoz-Valle, Jose F, Orozco-Barocio, Gerardo, Oregon-Romero, Edith, Navarro-Hernández, Rosa E, Salazar-Páramo, Mario, Armendariz-Borunda, Juan, Gámez-Nava, Jorge I, Gonzalez-Lopez, Laura, Chan, Jason Yf, Chan, Edward Kl, Satoh, Minoru
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01-01-2010; BioMed Central
• Subjects: Adult; Age of Onset; Autoantibodies; Autoantibodies - blood; Autoantibodies - immunology; Autoantigens - immunology; Blotting, Western; DEAD-box RNA Helicases - immunology; Distribution; Enzyme-Linked Immunosorbent Assay; Genetic aspects; Helicases; Humans; Immunoprecipitation; Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - immunology; Mexico; Middle Aged; Neoplasm Proteins - immunology; Prevalence; Research article; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Systemic lupus erythematosus; Mexico
• ISSN: 1478-6354; 1478-6362; 1478-6354
• DOI: 10.1186/ar2905

Autoantibodies to RNA helicase A (RHA) were reported as a new serological marker of systemic lupus erythematosus (SLE) associated with early stage of the disease. Anti-RHA and other autoantibodies in Mexican SLE patients and their correlation with clinical and immunological features were examined.
Autoantibodies in sera from 62 Mexican SLE patients were tested by immunoprecipitation of 35S-labeled K562 cell extract and enzyme-linked immunosorbent assay (anti-U1RNP/Sm, ribosomal P, beta2GPI, and dsDNA). Anti-RHA was screened based on the immunoprecipitation of the 140-kDa protein, the identity of which was verified by Western blot using rabbit anti-RHA serum. Clinical and immunological characteristics of anti-RHA-positive patients were analyzed.
Anti-RHA was detected in 23% (14/62) of patients, a prevalence higher than that of anti-Sm (13%, 8/62). Prevalence and levels of various autoantibodies were not clearly different between anti-RHA (+) vs. (-) cases, although there was a trend of higher levels of anti-RHA antibodies in patients without anti-U1RNP/Sm (P = 0.07). Both anti-RHA and -Sm were common in cases within one year of diagnosis; however, the prevalence and levels of anti-RHA in patients years after diagnosis did not reduce dramatically, unlike a previous report in American patients. This suggests that the high prevalence of anti-RHA in Mexican patients may be due to relatively stable production of anti-RHA.
Anti-RHA was detected at high prevalence in Mexican SLE patients. Detection of anti-RHA in races in which anti-Sm is not common should be clinically useful. Racial difference in the clinical significance of anti-RHA should be clarified in future studies.