Baccharis trimera improves the antioxidant defense system and inhibits iNOS and NADPH oxidase expression in a rat model of inflammation

• Published in: Current pharmaceutical biotechnology Vol. 14; no. 11; p. 975
• Main Authors: Cruz Padua, Bruno da, Rossoni Junior, Joamyr Victor, de Brito Magalhaes, Cíntia Lopes, Seiberf, Janaina Brandao, Araujo, Carolina Morais, Bianco de Souza, Gustavo Henrique, Chaves, Miriam Martins, Silva, Marcelo Eustaquio, Pedrosa, Maria Lucia, Costa, Daniela Caldeira
• Format: Journal Article
• Language: English
• Published: Netherlands 01-11-2013
• Subjects: Acetaminophen; Animals; Baccharis - chemistry; Cells, Cultured; Enzyme Activation - drug effects; Enzyme Activation - immunology; Immunity, Innate - drug effects; Immunity, Innate - immunology; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - immunology; Male; NADPH Oxidases - immunology; Neutrophil Activation - drug effects; Neutrophil Activation - immunology; Nitric Oxide Synthase Type II - antagonists & inhibitors; Nitric Oxide Synthase Type II - immunology; Plant Extracts - therapeutic use; Rats; Rats, Inbred F344; Reactive Oxygen Species - immunology; Treatment Outcome
• ISSN: 1873-4316
• DOI: 10.2174/1389201014666131226151728

Acetaminophen is a common analgesic and antipyretic compound which, when administered in high doses, has been associated with significant morbidity and mortality, secondary to hepatic toxicity. Although this may be due to a direct interaction of reactive acetaminophen metabolites with hepatocyte proteins, recent studies have suggested that reactive species produced by neutrophils also contribute to the pathophysiological process. Researches on the chemical composition of B. trimera show that this plant has bioactive compounds such as flavonoids, related to the organism's protection against free radicals. Therefore, in the present study, using Fischer rats, the effect of B. trimera on the antioxidant defense system, the production of nitric oxide (NO) and on the expression of nitric oxide synthase (iNOS), superoxide dismutase (SOD), catalase (CAT) and of the subunits of the NADPH oxidase in neutrophils was evaluated in a model of phagocytosis induced by zimosan (ZC3b) and in a model of inflammation induced by acetaminophen. The results show that the treatment with B. trimera improves the defense system of antioxidant and restores the balance ROS / NO that is altered in the inflammatory process induced by APAP. In conclusion, B. trimera extracts exert antioxidant properties by scavenging ROS and decrease the expression of genes responsible by reactive species production in neutrophils.