Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen

This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-...

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Bibliographic Details
Published in:Veterinary record Vol. 178; no. 10; p. 241
Main Authors: Roach, J., Schnobrich, M., Ellerbrock, R., Feijo, L., Bradecamp, E., Alvarenga, M. A., Kline, K., Canisso, I.
Format: Journal Article
Language:English
Published: England BMJ Publishing Group Limited 05-03-2016
Blackwell Publishing Ltd
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Summary:This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5°C for all groups. Sperm motility (total motility (%TM) and progressive motility (%PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, %TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in %TM or %PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P=0.03) and filter groups (P<0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving %TM and enhancing %PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge.
Bibliography:Provenance: not commissioned; externally peer reviewed
ObjectType-Article-1
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ISSN:0042-4900
2042-7670
DOI:10.1136/vr.103607