Dose-response Evaluation of Propolis Dental Varnish in Children: A Randomized Control Study

• Published in: Recent patents on biotechnology Vol. 14; no. 1; p. 41
• Main Authors: Neto, Edilson Martins Rodrigues, Valadas, Lídia Audrey Rocha, Lobo, Patrícia Leal Dantas, Fernandes, Analice Mendes Barreto, da Cruz Fonseca, Said Gonçalves, Fechine, Francisco Vagnaldo, Júnior, Francisco Josimar Girão, Bandeira, Mary Anne Medeiros, de França Fonteles, Marta Maria
• Format: Journal Article
• Language: English
• Published: United Arab Emirates 2020
• Subjects: Cariostatic Agents - administration & dosage; Cariostatic Agents - pharmacology; Cariostatic Agents - therapeutic use; Child; Child, Preschool; Dental Caries - prevention & control; Dose-Response Relationship, Drug; Female; Humans; Male; Patents as Topic; Pilot Projects; Propolis - administration & dosage; Propolis - pharmacology; Propolis - therapeutic use; Saliva - microbiology; Streptococcus mutans - drug effects; saliva; BRP varnish; dentistry; BRP extract; Propolis; Streptococcus mutans
• ISSN: 2212-4012
• DOI: 10.2174/1872208313666190826145453

Early childhood caries is a sugar-dependent disease with multifactorial modulating factors affecting deciduous dentition. It is defined as the presence of at least one decayed tooth, absence of a tooth due to caries or the existence of a temporary restoration in a tooth in a child between zero and 71 months of age. No BRP varnish was found in intellectual property banks, therefore it was registered and deposited with patent number BR1020160190142.
The objective of this study was to evaluate the dose-response concentration of alcoholic extract of Brazilian red propolis (BRP), in the form of dental varnish, against Streptococcus mutans (S. mutans) in children.
Twenty-four children, aged between 36 and 71 months, of both genders and without caries, were selected to participate in this pilot study and grouped randomly into four groups to receive different concentrations of BRP varnish (1%, 2.5%, 5% and 10%). The varnish was applied to the surface of all second deciduous molars. The antimicrobial activity was observed in saliva, which was collected in two phases: before applying the BRP varnish and after use.
There was microbiological reduction of S. mutans in the oral cavity of the children in all the tested concentrations. The highest percentage reduction of S. mutans was observed at the concentration of 2.5% (P = 0.0443).
The BRP extract in the form of dental varnish has antimicrobial activity against S. mutans and constitutes a possible alternative in the prevention of dental caries.