Fowl Adenovirus Serotype 4 Associated with Outbreaks of Infectious Hydropericardium in Haryana, India

• Published in: Avian diseases Vol. 46; no. 1; pp. 230 - 233
• Main Authors: Dahiya, Swati, Srivastava, Ravinder N, Hess, Michael, Gulati, Baldev R
• Format: Journal Article
• Language: English
• Published: United States American Association of Avian Pathologists, Inc 01-01-2002
• Subjects: Adenoviridae Infections - diagnosis; Adenoviridae Infections - epidemiology; Adenoviridae Infections - veterinary; Adenoviruses; Animals; Aviadenovirus; Aviadenovirus - classification; Aviadenovirus - isolation & purification; broiler chickens; Case Report; Case Reports; Cell culture techniques; Chickens; Disease outbreaks; Disease Outbreaks - veterinary; experimental infection; fowl adenovirus; heart diseases; hepatocytes; Inclusion bodies; India - epidemiology; Infectious diseases; infectious hydropericardium; Liver; Liver - virology; Mortality; Neutralization Tests - veterinary; outbreaks; PCR; Pericardial Effusion - epidemiology; Pericardial Effusion - veterinary; Pericardial Effusion - virology; pericardium; Polymerase chain reaction; Polymerase Chain Reaction - veterinary; Poultry Diseases - diagnosis; Poultry Diseases - epidemiology; Poultry Diseases - virology; serotyping; Serotyping - veterinary; Viruses; India
• ISSN: 0005-2086; 1938-4351
• DOI: 10.1637/0005-2086(2002)046[0230:FASAWO]2.0.CO;2

During 1998, hydropericardium syndrome was observed among 3-to-6-wk-old broilers in 45 different flocks of Haryana, India, with mortality ranging between 10% and 30%. Fowl adenovirus (FAV) was isolated from one of the affected flocks by chicken embryo liver cell culture. Serum neutralization test and polymerase chain reaction assay coupled with restriction enzyme analysis confirmed that the isolated virus belonged to FAV serotype 4. The disease was reproduced in 28-day-old broilers by subcutaneous and oral inoculation of isolated FAV4 alone. Typical hydropericardium and basophilic intranuclear inclusions in hepatocytes were observed in experimental birds by day 4 postinoculation.