Lipopolysaccharide-induced chorioamnionitis causes acute inflammatory changes in the ovine central nervous system

• Published in: CNS & neurological disorders drug targets Vol. 14; no. 1; p. 77
• Main Authors: Strackx, Eveline, Sparnaaij, Michelle A R, Vlassaks, Evi, Jellema, Reint, Kuypers, Elke, Vles, Johan S H, Kramer, Boris W, Gavilanes, Antonio W D
• Format: Journal Article
• Language: English
• Published: United Arab Emirates 01-01-2015
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - physiology; Catalase - genetics; Catalase - metabolism; Central Nervous System - embryology; Central Nervous System - metabolism; Central Nervous System - pathology; Chorioamnionitis - chemically induced; Chorioamnionitis - physiopathology; Chorioamnionitis - veterinary; Cytokines - genetics; Cytokines - metabolism; Female; Fetus; Gene Expression Regulation, Developmental - drug effects; Inflammation - etiology; Inflammation - pathology; Inflammation - veterinary; Lipopolysaccharides - toxicity; Pregnancy; RNA, Messenger - metabolism; Sheep; Time Factors; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism
• ISSN: 1996-3181
• DOI: 10.2174/1871527314666150116120029

To better understand the inflammatory response in the central nervous system (CNS) after lipopolysaccharide (LPS)-induced chorioamnionitis.
Fetal sheep were exposed to intra-amniotic LPS 2 or 14 days before preterm delivery at 125 days of gestation. mRNA levels of cytokines, TLRs and anti-oxidants were determined in different CNS regions.
Interleukin 1β levels increased in hippocampus, cortex and cerebellum 2 days after LPS exposure, while Interleukin 8 levels increased in the periventricular white matter as well. Levels returned back to control levels after 14 days. Tumor necrosis factor-α levels increased in hippocampus and cortex after 2 days. Toll like receptor 4 levels was upregulated in all grey matter regions 2 and 14 days after exposure. Glutathione s-transferase mRNA levels were lower after 2 and 14 days in all grey matter regions.
Intra-amniotic LPS exposure causes acute and region-specific changes in inflammatory markers in the fetal brain, with grey matter being more affected than white matter.
Intra-amniotic LPS exposure causes acute and region-specific changes in cytokines, TLR and anti-oxidants levels, with grey matter being more affected than white matter.