Monoclonal antibody enhancement of FSH-induced uterine growth in snell dwarf mice

• Published in: Journal of endocrinology Vol. 136; no. 3; p. R5
• Main Authors: Glencross, R G, Lovell, R D, Holder, A T
• Format: Journal Article
• Language: English
• Published: England 01-03-1993
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - pharmacology; Cattle; Dwarfism, Pituitary - physiopathology; Female; Follicle Stimulating Hormone - immunology; Follicle Stimulating Hormone - pharmacology; Mice; Mice, Mutant Strains; Organ Size - drug effects; Uterus - anatomy & histology; Uterus - drug effects; Uterus - growth & development
• ISSN: 0022-0795
• DOI: 10.1677/joe.0.136R005

The enhancing effect of bovine FSH monoclonal antibody (bFSH-MAb) on the gonadotrophic activity of FSH was investigated in dwarf mice using a uterine weight bioassay. Increasing doses of bovine FSH (NIH-FSH-B1; 3.3, 10 or 30 micrograms/day) were administered for 5 days to dwarf mice (groups of five) with or without administration of a bFSH-MAb preparation (USDA-bFSH-MC28; 100 micrograms protein/day) which at a dilution of 1:15,000 bound 50% of 125I-labelled bFSH (USDA-bFSH-BP3). The bFSH, at the doses given, gave no increases in uterine weight; when, however, these doses were given with bFSH-MAb, significant (three- to four fold) increases in uterine weight resulted.