Association of inhibitor of differentiation 1 expression with human papillomaviruses infections in cervical carcinoma

• Published in: International journal of gynecological cancer Vol. 21; no. 7; p. 1276
• Main Authors: Li, Jinke, Xie, Lingxia, Gan, Xiaoling, Liu, Bao, Zhang, Yi, Song, Bo, Lin, Yong, Hu, Lina
• Format: Journal Article
• Language: English
• Published: England 01-10-2011
• Subjects: Adult; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - virology; Female; Genotype; Human papillomavirus 16 - genetics; Humans; Inhibitor of Differentiation Protein 1 - metabolism; Middle Aged; Papillomavirus Infections - complications; Papillomavirus Infections - metabolism; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - virology
• ISSN: 1525-1438
• DOI: 10.1097/IGC.0b013e31821f7452

Our recent study has shown that inhibitor of differentiation 1 (Id-1) is overexpressed in cervical carcinoma. However, the relationship between Id-1 expression and human papillomavirus (HPV) infection has not been elucidated. In this study, we investigate the association of Id-1 protein expression and HPV infection in cervical carcinoma tissues.
A total of 56 paraffin-embedded and 12 fresh cervical carcinoma tissues were collected for Id-1 and HPV detection. The Id-1 protein was detected by immunohistochemistry and Western blot in paraffin-embedded and fresh carcinoma tissues. Human papillomavirus DNA was detected and genotyped by using an oligonucleotide microarray and polymerase chain reaction.
The overall HPV prevalence was 82.1%, whereas that of HPV type 16 (HPV-16) was 62.5% in cervical carcinoma. The HPV-positive samples showed higher Id-1 expression levels than the HPV-negative ones (Cochran-Mantel-Haenszel χ test [χCMH] = 4.39, P < 0.05). The HPV-16-infected samples had higher Id-1 expression levels than the samples with infection of other single HPV genotypes (χCMH = 6.42, P < 0.02). The results of Western blot were correlated to the immunohistochemistry results, showing a higher Id-1 expression level in HPV-infected especially HPV-16-infected carcinoma tissues.
Inhibitor of differentiation 1 expression is correlated to HPV infection in cervical carcinoma, suggesting that Id-1 plays a role in HPV-related cervical carcinogenesis.