THE SEVERITY OF WHIRLING DISEASE AMONG WILD TROUT CORRESPONDS TO THE DIFFERENCES IN GENETIC COMPOSITION OF TUBIFEX TUBIFEX POPULATIONS IN CENTRAL COLORADO

• Published in: The Journal of parasitology Vol. 91; no. 1; pp. 53 - 60
• Main Authors: Beauchamp, Katherine A, Kelley, Garry O, Nehring, R. Barry, Hedrick, Ronald P
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01-02-2005; Allen Press Inc
• Subjects: Animal diseases; Animals; Biological and medical sciences; Colorado; Disease reservoirs; disease severity; Disease susceptibility; DNA, Ribosomal - chemistry; Electrophoresis, Agar Gel - veterinary; Epidemiology; Fish Diseases - parasitology; Fish Diseases - transmission; Fish populations; Fresh Water; FUNCTIONAL MORPHOLOGY; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; genetic markers; genetic resistance; Genetic screening; genetic variation; Genotypes; Geographical distribution; geographical variation; Global positioning systems; GPS; Habitats; Infections; intermediate hosts; Invertebrates; Laboratories; Mitochondrial DNA; Myxobolus cerebralis; Natural reservoirs; Oligochaeta - classification; Oligochaeta - genetics; Oligochaeta - parasitology; Oncorhynchus mykiss; Parasites; Parasitic diseases; Phylogenetics; Polymerase Chain Reaction - veterinary; population genetics; Populations; Protozoan Infections, Animal - parasitology; Protozoan Infections, Animal - transmission; Ribosomal DNA; Rivers; RNA, Ribosomal, 16S - genetics; rRNA 16S; Tall tales; Trout; Trout - parasitology; Tubifex tubifex; Water use; Whirling disease; Worms; Colorado; United States; North America; America; Montana; United States > US; Europe; Colorado River; South Platte River; California; Tubifex tubifex; Composition; Disease; Parasite; Genetics; Invertebrata; Annelida; Oligochaeta
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-327R

We analyzed the geographic distribution of Tubifex tubifex from various river drainages in central Colorado by genetic screening with specific mitochondrial 16S ribosomal DNA (mt 16S rDNA) markers. Four distinct mt 16S rDNA lineages are evident. The sites varied with respect to land- and water-use practices. All sites represented habitats presumed capable of supporting oligochaetes. At the locations where whirling disease has had the greatest impact on resident rainbow trout, T. tubifex, representing lineages I and III (genotypes known to be susceptible to Mxyobolus cerebralis), were most commonly found. In contrast, at sites less affected by whirling disease, T. tubifex of lineages V and VI that are more resistant to M. cerebralis infections were more abundant. The predominance of resistant T. tubifex worms (lineages V and VI) at low-impact sites supports the conclusion that when these genotypes are in greater abundance, the potential for more severe effects of whirling disease on wild rainbow trout populations may be diminished.