Determining the Effectiveness of Three-Gene Pyramids Against Aphis glycines (Hemiptera: Aphididae) Biotypes

• Published in: Journal of economic entomology Vol. 110; no. 6; pp. 2428 - 2435
• Main Authors: Varenhorst, A. J, Pritchard, S. R, O'Neal, M. E, Hodgson, E. W, Singh, A. K
• Format: Journal Article
• Language: English
• Published: US Entomological Society of America 05-12-2017; Oxford University Press
• Subjects: Agrochemicals; Animals; Aphididae; Aphids - physiology; Aphis glycines; avirulent; Biotypes; Cultivars; FIELD AND FORAGE CROPS; Gene frequency; Genes; Glycine max - genetics; Glycine max - growth & development; Hemiptera; Herbivory; host plant resistance; Host plants; Insecticide resistance; Insecticides; Management; Pest Control, Biological - methods; Phenotype; Plant Breeding; Plant resistance; Rag genes; RAG1 protein; RAG2 protein; soybean aphid; Sustainable use; virulent; United States > US; virulent; host plant resistance; avirulent; genes; soybean aphid; Rag genes
• ISSN: 0022-0493; 1938-291X
• DOI: 10.1093/jee/tox230

Since the discovery of Aphis glycines Matsumura (Hemiptera: Aphididae) in the United States, the primary management tactic has been foliar insecticides. Alternative management options such as host plant resistance to A. glycines have been developed and their effectiveness proved. However, the use of host plant resistance was complicated by the discovery of multiple, virulent biotypes of A. glycines in the United States that are capable of overcoming single Rag genes, Rag1 and Rag2, as well as a two-gene pyramid of Rag1+Rag2. However, current models predict that the virulent allele frequency of A. glycines decreases in response to the use of pyramided Rag genes, suggesting that pyramids represent a more sustainable use of these traits. Previous research has demonstrated that virulent biotypes can be effectively managed using a three-gene pyramid of Rag1+Rag2+Rag3. Additional Rag-genes have been discovered (Rag4 and Rag5), but whether the incorporation of these genes into novel three-gene pyramids will improve efficacy is not known. We tested single-gene (Rag1 and Rag2) and pyramid cultivars (Rag1+Rag2, Rag1+Rag2+Rag3, Rag1+Rag2+Rag4) to multiple biotypes in laboratory assays. Our results confirm that the Rag1+Rag2+Rag3 pyramid effectively manages all known A. glycines biotypes when compared with cultivars that are overcome by the associated biotype. Our results indicate that Rag1+Rag2+Rag4 would be an effective management option for biotype-1, biotype-2, and biotype-3 A. glycines, but had a negligible impact on biotype-4.