Regulation of COX-2 transcription in a colon cancer cell line by Pontin52/TIP49a

• Published in: Molecular cancer Vol. 2; no. 1; p. 42
• Main Authors: Carlson, Mary L, Wilson, Ellen T, Prescott, Stephen M
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15-12-2003; BioMed Central; BMC
• Subjects: ATPases Associated with Diverse Cellular Activities; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell Line, Tumor; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - metabolism; Cell Transformation, Neoplastic - pathology; Colonic Neoplasms - enzymology; Colonic Neoplasms - genetics; Colonic Neoplasms - pathology; Cyclooxygenase 2; DNA Helicases - genetics; DNA Helicases - metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; In Situ Hybridization; Membrane Proteins; Prostaglandin-Endoperoxide Synthases - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Short Communication; Transcription, Genetic - genetics
• ISSN: 1476-4598; 1476-4598
• DOI: 10.1186/1476-4598-2-42

Cyclooxygenase-2 (COX-2) is expressed early in colon carcinogenesis and is known to play a crucial role in the progress of the disease. Here we show that the regulation of the expression of this enzyme in a colon cancer cell line, and in patients, is associated with overexpression of the Wnt pathway-associated proteins, Pontin52/TIP49a and LEF-1. Recently shown to be essential for transformation via the c-Myc pathway, Pontin52/TIP49a promotes COX-2 expression in tissue culture and is overexpressed in colon cancer tissue, co-localizing with COX-2 expression in transformed tissue, relative to paired normal tissue.