Detection of Helicobacter pylori cagA gene in gastric biopsies, clinical isolates and faeces

• Published in: Indian journal of medical microbiology Vol. 24; no. 3; pp. 195 - 200
• Main Author: Bindayna KM, Al Baker WA, Botta GA
• Format: Journal Article
• Language: English
• Published: India Medknow Publications on behalf of Indian Association of Medical Microbiology 01-07-2006; Elsevier B.V
• Subjects: Adolescent; Adult; Aged; Antigens, Bacterial - genetics; Antigens, Bacterial - isolation & purification; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bahrain; Biopsy; CagA gene; CagA gene, gastric biopsy, Helicobacter pylori, stool; DNA, Bacterial - isolation & purification; Feces - microbiology; Female; gastric biopsy; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Humans; Male; Middle Aged; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Stomach - microbiology; stool; Bahrain; stool; Helicobacter pylori; gastric biopsy; CagA gene
• ISSN: 0255-0857
• DOI: 10.1016/S0255-0857(21)02349-5

Purpose: Helicobacter pylori infection is common in the developing
countries. The cagA gene is a marker of pathogenicity island (PAI) in
H. pylori . The aim of this study was to determine the prevalence of
cagA among dyspeptic patients in Bahrain directly from gastric biopsy
and stool specimen. Methods: A total of 100 gastric biopsy samples,
16 clinical isolates and 44 faecal specimens were collected from
Bahraini adult dyspeptic patients. cagA gene of H. pylori was
assessed using polymerase chain reaction (PCR). Results: The cagA
gene was detected in 59 (59%) from biopsy specimens, 10 (62%) clinical
isolates and in 10 (22.7%) faecal specimens. The detection of cagA
positive H. pylori was significantly higher in patients with duodenal
ulcer (80%) compared to those with other endoscopic finding (42%) ( P
< 0.05). Conclusions: Using PCR to detect cagA gene directly from
biopsy is a rapid and reliable technique. However, using stool specimen
for genotyping in our patients showed reduced sensitivity.