Protective effect of Lactiplantibacillus plantarum LP5 in a murine model of colonisation by Campylobacter coli DSPV458

• Published in: Beneficial microbes Vol. 12; no. 6; p. 553
• Main Authors: Ruiz, M J, Sirini, N E, Signorini, M L, Etcheverría, A, Zbrun, M V, Soto, L P, Zimmermann, J A, Frizzo, L S
• Format: Journal Article
• Language: English
• Published: Netherlands 16-11-2021
• Subjects: Animals; Campylobacter coli; Campylobacter Infections - therapy; Colony Count, Microbial; Disease Models, Animal; Feces; Female; Intestines - microbiology; Lactobacillus plantarum; Mice; Mice, Inbred BALB C; Probiotics - therapeutic use; Swine; in vivo assay; antibiotic alternative; lactic acid bacteria; Campylobacter coli; food-borne pathogen
• ISSN: 1876-2891
• DOI: 10.3920/BM2021.0010

Thermotolerant
species are the leading cause of foodborne bacterial diarrheal disease worldwide.
, abundant in pigs and pork products, have been identified as a source of human infection. In this study, we propose the use of
LP5 as a probiotic to reduce colonisation of this intestinal pathogen in a murine colonisation model of
DSPV458. Six-week-old adult female
mice were housed in groups: Control, Campy and Pro-Campy. Control and Pro-Campy groups received antibiotics for 5 days and the Campy group for 12 days. Pro-Campy group was inoculated for 7 days with 8.78 log
cfu total of
LP5 suspended in De Man, Rogosa and Sharpe broth. All groups were inoculated with 6.72 log
cfu of
DSPV458 suspended in brain heart infusion broth.
LP5 was recovered only in the Pro- Campy group.
DSPV458 was recovered at higher levels in the Control and Campy groups. The differences with the Pro-Campy group were significant. As regards faeces, Control and Campy groups reached 7.41 and 7.84 log
cfu/g, respectively, and the Pro-Campy group only 4.62 log
cfu/g. In the caecum, Control and Campy groups reached 8.01 and 9.26 log
cfu/g, respectively, and the Pro-Campy group only 4.51 log
cfu/g. In the ileum, Control and Campy groups reached 3.43 and 3.26 log
cfu/g, respectively, and the Pro-Campy group did not show detectable levels. The reduction of
DSPV458 in the Pro-Campy group compared to the Control group in faeces, caecum and ileum was 99.55, 99.98 and 100%, respectively. Animals were maintained under normal health conditions, and haematological parameters were within the standard values for
. The incorporation of a probiotic generated a protective effect in the mice colonisation model. The protective effect would also apply to intestinal colonisation by indigenous enterobacteria. Therefore, the strategy used in this study is of great importance to understand the protection mechanisms in a murine model, as well as its application in food-producing animals.