Daphnane diterpene hirsein B downregulates melanogenesis in B16 murine melanoma cells by cAMP pathway inhibition

Daphnane diterpene hirsein B downregulates melanogenesis in B16 murine melanoma cells by cAMP pathway inhibition

Materials and methods Total RNA was isolated from B16 murine melanoma cells (Riken Cell Bank, Tsukuba, Japan) and used for DNA microarray analysis, using chips of 528 spots loaded with 265 genes prepared by Genopal(TM) (Mitsubishi Rayon Co., Ltd, Tokyo, Japan), to determine the expressions of genes...

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Bibliographic Details
Published in:BMC proceedings Vol. 5 Suppl 8; no. S8; p. P68
Main Authors: Villareal, Myra O, Han, Junkyu, Ikuta, Kenjiro, Isoda, Hiroko
Format: Journal Article
Language:English
Published: England BioMed Central 22-11-2011
BioMed Central Ltd
Subjects:
Colleges & universities
Gene expression
Kinases
Meeting Abstract
Meetings
Pituitary gland
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Summary:Materials and methods Total RNA was isolated from B16 murine melanoma cells (Riken Cell Bank, Tsukuba, Japan) and used for DNA microarray analysis, using chips of 528 spots loaded with 265 genes prepared by Genopal(TM) (Mitsubishi Rayon Co., Ltd, Tokyo, Japan), to determine the expressions of genes for melanogenesis, membrane-bound receptors, tyrosine kinase regulation, melanosome transport, and other cell signal regulation-related genes (including the housekeeping and negative control genes).
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ISSN:1753-6561
1753-6561
DOI:10.1186/1753-6561-5-S8-P68