Stimulation of IL-8 release from epithelial cells by gas cooker PM10: a pilot study

• Published in: Occupational and environmental medicine (London, England) Vol. 58; no. 3; pp. 208 - 210
• Main Authors: Dick, C A J, Dennekamp, M, Howarth, S, Cherrie, J W, Seaton, A, Donaldson, K, Stone, V
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd 01-03-2001; BMJ Publishing Group; BMJ; BMJ Publishing Group LTD; BMJ Group
• Subjects: Air; Air pollution; Biological and medical sciences; Cell lines; Cooking; Cultured cells; Cytokines; Cytotoxicity; Dioxides; Environmental pollutants toxicology; Epithelial cells; Gases; Gene expression; Indoor air pollution; interleukin-8; Laboratories; Lungs; Medical sciences; Nitrogen; Nitrogen dioxide; Outdoor air quality; Oxidative stress; Particulate matter; PM10; Short Report; Studies; Toxicology; Variance analysis; Human; Cell culture; Toxicity; Lung; Domestic kitchen; Cytokine; Respiratory system; In vitro; Particle; Indoor pollution; Epithelial cell; Air pollution; Nitrogen dioxide; Interleukin 8
• ISSN: 1351-0711; 1470-7926
• DOI: 10.1136/oem.58.3.208

OBJECTIVE To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2). METHOD Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. RESULTS Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. CONCLUSION PM10generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.