PLANT REGENERATION OF AGAVE TEQUILANA BY INDIRECT ORGANOGENESIS

• Published in: In vitro cellular & developmental biology. Plant Vol. 42; no. 4; pp. 336 - 340
• Main Authors: VALENZUELA-SÁNCHEZ, KARLA K., JUÁREZ-HERNÁNDEZ, RAUL E., CRUZ-HERNÁNDEZ, ANDRÉS, OLALDE-PORTUGAL, VÍCTOR, ELENA VALVERDE, MARÍA, PAREDES-LÓPEZ, OCTAVIO
• Format: Journal Article
• Language: English
• Published: Heidelberg CABI Publishing 01-07-2006; Springer Nature B.V
• Subjects: Agavaceae; Auxins; Callus; callus culture; Cytokinins; Developmental Biology/Morphogenesis; meristem; Meristems; Organogenesis; Plant cells; Plant propagation; Plantlets; Plants; Regeneration; tissue culture
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1079/IVP2006788

Indirect organogenesis was developed in Agave tequilana. Leaf segments and meristematic tissue from the central head (‘piña’) were evaluated as explant sources. A minimal-sized explant with high bud-forming capacity (19.5 BFC) was obtained through a cross section of meristematic tissue from in vitro plantlets. In callus culture, the best growth response was due to naphthalene acetic-acid (NAA) presenting a contrasting response compared to 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration from meristem segments and callus was obtained using 1.1 μM 2,4-D and 44 μM 6-benzylaminopurine (BA). The regeneration capacity of callus was maintained for 3 mo. Shoots regenerated were rooted in a hormone-free MS1 medium and acclimatized in a greenhouse with a 100% survival.