Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

• Published in: Electronic Journal of Biotechnology Vol. 10; no. 1
• Main Authors: Lakshmanan, Venkatachalam, Venkataramareddy, Sreedhar Reddampalli, Neelwarne, Bhagyalakshmi
• Format: Journal Article
• Language: English
• Published: Universidad Católica de Valparaíso 15-01-2007
• Subjects: Growth regulators, Musa, protocol, shoot cultures, somaclonal variation
• ISSN: 0717-3458; 0717-3458
• DOI: 10.2225/vol10-issue1-fulltext-12

A large number of micropropagated plantlets of banana, Musa acuminata
var. Nanjanagudu Rasabale (NR), that were developed from axillary
shoot bud explants over 10 years ago were screened for genetic
variation, if any, using RAPD (Random Amplified Polymorphic DNA) and
ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro
plantlets, 11 were used for screening that involved shoot cultures with
distinct variation in morphological characteristics (morphotypes).
Similarly, the mother maintained in the field was also subjected for
genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30
RAPD and 5 ISSR primers produced totally 424 clear, distinct and
reproducible band classes resulting in a total of 5088 bands where the
banding patterns for each primer was highly uniform and comparable to
the field-grown mother clone from which the cultures had been
established. These results indicate that the micropropagation protocol
developed by us for rapid in vitro multiplication is appropriate and
applicable for clonal propagation of banana var. NR over a long period.
This is the first report on the use of genetic markers to establish
genetic fidelity of long-term micropropagated banana using RAPD and
ISSR.