Antiproliferative Activity of Some Medicinal Plants on Human Breast and Hepatocellular Carcinoma Cell Lines and their Phenolic Contents

• Published in: Tropical journal of pharmaceutical research Vol. 14; no. 10; p. 1787
• Main Authors: Karakas, Fatma Pehlivan, Yildirim, Arzu Birinci, Bayram, Recep, Yavuz, Muhsine Z, Gepdiremen, Akcahan, Turker, Arzu Ucar
• Format: Journal Article
• Language: English
• Published: Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria 01-10-2015
• Subjects: Antiproliferative; Bellis perennis; Breast cancer; Convolvulus galaticus; HepG2/C3A; Lysimachia vulgaris; MCF-7; Phenolics; Trifolium pannonicum subsp. elongatum
• ISSN: 1596-5996; 1596-9827
• DOI: 10.4314/tjpr.v14i10.8

Purpose: To determine the phenolic composition and antiproliferative
activity of 16 different extracts (hexane, dichloromethane, methanol
and water) obtained from Bellis perennis , Convolvulus galaticus ,
Trifolium pannonicum and Lysimachia vulgaris on human breast cancer
(MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines.
Methods: The aerial parts of the plants were successively extracted
with hexane, dichloromethane, methanol and water using a Soxhlet
apparatus. The phenolic content of the plants were determined by plants
by high performance liquid chromatography (HPLC) while their
antiproliferative activity was evaluated by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow
tetrazole (MTT) assay. Results: Among the tested extracts, the methanol
extract of B. perennis showed the best antiproliferative activity
against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth)
value of 71.6 μg/mL. Furthermore, the dichloromethane extract of
C. galaticus showed the best anti-proliferative activity against
HepG2/C3A cell line with IC50 of 57.3 μg/mL. The HPLC data for the
plant extracts showed the presence of the following phenolic compounds:
gallic acid monohydrate, caffeic acid, rutin hydrate,
luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin,
coumarin and apigenin. Conclusion: The findings of this study indicate
that there is some justification for the use of B. perennis and C.
galaticus as traditional anticancer medicinal herbs.