Development and Characterization of EST-SSR Markers for Ottelia acuminata var. jingxiensis (Hydrocharitaceae)

• Published in: Applications in plant sciences Vol. 5; no. 11
• Main Authors: Li, Zhi-Zhong, Lu, Meng-Xue, Saina, Josphat K, Gichira, Andrew W, Wang, Qing-Feng, Chen, Jin-Ming
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01-11-2017
• Subjects: expressed sequence tag–simple sequence repeat (EST-SSR); Hydrocharitaceae; microsatellite; Ottelia acuminata var. jingxiensis; PRIMER NOTES; transcriptome; Ottelia acuminata var. jingxiensis; microsatellite; Hydrocharitaceae; transcriptome; expressed sequence tag–simple sequence repeat (EST-SSR)
• ISSN: 2168-0450; 2168-0450
• DOI: 10.3732/apps.1700083

Premise of the study: Simple sequence repeat (SSR) markers were derived from transcriptomic data for Ottelia acuminata (Hydrocharitaceae), a species comprising five endemic and highly endangered varieties in China. Methods and Results: Sixteen novel SSR markers were developed for O. acuminata var. jingxiensis. One to eight alleles per locus were found, with a mean of 2.896. The observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.793, respectively. Interestingly, in cross-varietal amplification, 13 out of the 16 loci were successfully amplified in O. acuminata var. acuminata, and 12 amplified in each of the other three varieties of O. acuminata. Conclusions: These newly developed SSR markers will facilitate further study of genetic variation and provide important genetic data needed for appropriate conservation of natural populations of all varieties of O. acuminata.