Differences of IL-1β Receptors Expression by Immunocompetent Cells Subsets in Rheumatoid Arthritis

• Published in: Mediators of Inflammation Vol. 2015; no. 2015; pp. 1006 - 1014-493
• Main Authors: Sennikov, Sergey V., Karaulov, Aleksander V., Sizikov, Aleksey E., Chumasova, Oksana A., Shkaruba, Nadezhda S., Anatolievna, Lopatnikova Julia, Alshevskaya, Alina A., Kozlov, Vladimir A.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Limiteds 01-01-2015; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Adolescent; Adult; Aged; Arthritis, Rheumatoid - metabolism; B-Lymphocytes - metabolism; Female; Flow Cytometry; Gene expression; Genetic aspects; Health aspects; Humans; Interleukin-1beta - metabolism; Interleukins; Male; Middle Aged; Monocytes - metabolism; Observations; Receptors, Interleukin-1 - metabolism; Rheumatoid arthritis; T-Lymphocytes - metabolism; Young Adult
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/2015/948393

IL-1β is involved in the induction and maintenance of chronic inflammation in rheumatoid arthritis (RA). Its activity is regulated and induced by soluble and membrane-bound receptors, respectively. The effectiveness of the cytokine depends not only on the percentage of receptor-positive cells in an immunocompetent subset but also on the density of receptor expression. The objective of this study was to investigate the expression of IL-1β membrane-bound receptors (IL-1R1 and IL-1R2) in terms of the percentage of receptor-positive cells and the number of receptors per cell in different subsets of immune cells in RA patients before and after a course of basic (excluding anticytokine) therapy and in healthy individuals. The resulting data indicate differences in the expression of IL-1β receptors among T cells, B cells, and monocytes in healthy volunteers and in rheumatoid arthritis patients. The importance of determining both the relative percentage of cells expressing receptors to immunomodulatory cytokines and the number of membrane-bound receptors per cell is highlighted by evidence of unidirectional or multidirectional changing of these parameters according to cell subset and health status.