Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone)

• Published in: Journal of Traditional and Complementary Medicine Vol. 9; no. 1; pp. 24 - 37
• Main Authors: Kaleeswaran, B., Ramadevi, S., Murugesan, R., Srigopalram, S., Suman, T., Balasubramanian, T.
• Format: Journal Article
• Language: English
• Published: Netherlands 國立臺灣大學食品與生物分子研究中心 01-01-2019; Elsevier Taiwan LLC; Elsevier
• Subjects: Kidney stone; Magnesium oxide; Original; Pedalium murex; Struvite crystal; Urolithiasis; Pedalium murex; Urolithiasis; Magnesium oxide; Kidney stone; Struvite crystal; MgO; EAEP; MBC; MBC, Minimum Bactericidal Concentration; MgO, Magnesium Oxide; EAEP, Ethyl acetate extract of Pedalium murex L
• ISSN: 2225-4110; 2225-4110
• DOI: 10.1016/j.jtcme.2017.08.003

Pedalium murex (L.) is a traditional herb, commonly used for the treatment of kidney stone related problems. Struvite stone can swiftly grow and become 'staghorn calculi' in kidney and its associated areas, which is the most aching urological disorder. The present study investigated the anti-urolithiasis activities of ethyl acetate extract of P. murex L. (EAEP) against struvite crystal. The antibacterial activity of EAEP examined against several urease producing bacteria. It showed the minimum bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus (＞125). On the other hand, total mass, volume, number, growth rate and dissolution rate of synthesised struvite crystals were observed at different concentrations 0.5%, 0.75%, 1% of EAEP and without EAEP. In which, EAEP addition showed appreciably reduced struvite crystal. Alternatively, MgO (300 mg of EAEP/kg/body weight) induced urolithiasis of Wistar albino rat at the rate of 1 ml for 28 days. Various biochemical parameters in serum, urine and histological analysis of kidney were taken for evaluation. Significant results (p ＜ 0.05) were observed in 1% EAEP (300 mg) treated group than cystone treated group. From the histological study, reduced renal damage and glomerular development were observed. Our experiment, P. murex L. enhances the reducing activity on struvite crystal and prevents the crystal formation both in-vitro and in-vivo. It can be suggesting that P. murex L. and its phyto-components could be used as remedy for the management of kidney stone by dissolving the struvite stone in kidney.