Chemical Composition and Antibacterial Activity of Essential Oils of Tagetes minuta (Asteraceae) against Selected Plant Pathogenic Bacteria

• Published in: International Journal of Microbiology Vol. 2016; no. 2016; pp. 262 - 270
• Main Authors: Wanzala, Wycliffe, Dossaji, Saifuddin F., Wagacha, John M., Gakuubi, Martin Muthee
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Limiteds 2016; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Antimicrobial agents; Asteraceae; Bacteria; Chemical composition; Compositae; Distillation; Essences and essential oils; Essential oils; Food supply; Gas chromatography; Health aspects; Hydrocarbons; Mass spectrometry; Microbiology; Monoterpenes; Observations; Oils & fats; Pathogens; Pesticides; Plant diseases; Plant-pathogen relationships; Pseudomonas savastanoi; Science; Studies; Tagetes minuta; Xanthomonas axonopodis
• ISSN: 1687-918X; 1687-9198
• DOI: 10.1155/2016/7352509

The objective of this study was to determine the chemical composition and antibacterial activity of essential oils (EOs) of Tagetes minuta against three phytopathogenic bacteria Pseudomonas savastanoi pv. phaseolicola, Xanthomonas axonopodis pv. phaseoli, and Xanthomonas axonopodis pv. manihotis. The essential oils were extracted using steam distillation method in a modified Clevenger-type apparatus while antibacterial activity of the EOs was evaluated by disc diffusion method. Gas chromatography coupled to mass spectrometry (GC/MS) was used for analysis of the chemical profile of the EOs. Twenty compounds corresponding to 96% of the total essential oils were identified with 70% and 30% of the identified components being monoterpenes and sesquiterpenes, respectively. The essential oils of T. minuta revealed promising antibacterial activities against the test pathogens with Pseudomonas savastanoi pv. phaseolicola being the most susceptible with mean inhibition zone diameters of 41.83 and 44.83 mm after 24 and 48 hours, respectively. The minimum inhibitory concentrations and minimum bactericidal concentrations of the EOs on the test bacteria were in the ranges of 24–48 mg/mL and 95–190 mg/mL, respectively. These findings provide a scientific basis for the use of T. minuta essential oils as a botanical pesticide for management of phytopathogenic bacteria.