Microfluidic Immunoassays for Sensitive and Simultaneous Detection of IgG/IgM/Antigen of SARS-CoV‑2 within 15 min

• Published in: Analytical chemistry (Washington) Vol. 92; no. 14; pp. 9454 - 9458
• Main Authors: Lin, Qiuyuan, Wen, Donghua, Wu, Jing, Liu, Liling, Wu, Wenjuan, Fang, Xueen, Kong, Jilie
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21-07-2020
• Subjects: Antibodies; Antibodies, Viral - analysis; Antigens; Betacoronavirus - immunology; Chemistry; Clinical Laboratory Techniques - methods; Coronavirus Infections - diagnosis; COVID-19; COVID-19 Testing; Enzyme-Linked Immunosorbent Assay; Health problems; Humans; Immunoassay; Immunoassay - methods; Immunoassays; Immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulin M; Immunoglobulin M - analysis; Letter; Microfluidic Analytical Techniques - methods; Microfluidics; Pandemics; Pharynx; Pharynx - chemistry; Pharynx - immunology; Pneumonia, Viral - diagnosis; Public health; SARS-CoV-2; Sensitivity and Specificity; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral diseases
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.0c01635

The outbreak of SARS-CoV-2 is posing serious global public health problems. Facing the emergence of this pandemic, we established a portable microfluidic immunoassay system for easy-to-use, sensitive, rapid (<15 min), multiple, and on-site detection of IgG/IgM/Antigen of SARS-CoV-2 simultaneously. This integrated method was successfully applied for detecting SARS-CoV-2 IgM and IgG antibodies in clinical human serum as well as SARS-CoV-2 antigen in pharyngeal swabs from 26 patients with COVID-19 infection and 28 uninfected people. The assay demonstrated high sensitivity and specificity, which is promising for the diagnosis and monitoring as well as control of SARS-CoV-2 worldwide.