Acute effect of hemodialysis on serum levels of the proinflammatory cytokines

• Published in: Mediators of Inflammation Vol. 2003; no. 1; pp. 15 - 19
• Main Authors: Tarakçioğlu, Mehmet, Erbağci, Ayşe Binnur, Usalan, Celalettin, Deveci, Rukiye, Kocabaş, Ramazan
• Format: Journal Article
• Language: English
• Published: United States Hindawi Limiteds 01-02-2003; Hindawi Limited
• Subjects: Adult; Blood Proteins - analysis; Clearance; Cytokines - blood; Female; Hemodialysis; Humans; Inflammation - etiology; Inflammation - immunology; Interleukin-1 - blood; Interleukin-6 - blood; Interleukin-8; Interleukin-8 - blood; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - complications; Kidney Failure, Chronic - therapy; Male; Middle Aged; Proinflammatory cytokines; Receptors, Interleukin-2 - blood; Regression Analysis; Renal Dialysis - adverse effects; Serum Albumin - analysis; Soluble interleukin-2 receptor; Tumor Necrosis Factor-alpha - analysis; Soluble interleukin-2 receptor; Proinflammatory cytokines; Clearance; Hemodialysis; Interleukin-8
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1080/0962935031000096935

Chronic inflammation is a common feature of end-stage renal disease, which carries a heightened risk of atherosclerosis and other co-morbid conditions. Dialysis treatment per se can bring additional risk factors for inflammation, such as increased risk of local graft and fistula infections, impure dialysate or bio-incompatible membranes. Our study was designed to determine whether a hemodialysis session leads to an acute substantial alteration in the plasma levels of the proinflammatory cytokines interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α, the T-lymphocyte activation factor soluble IL-2 receptor (sIL-2R), and an inflammation mediator and chemotactic granulocyte factor, IL-8, in end-stage renal disease patients receiving chronic intermittent HD. In this study, 21 (12 male/nine female) patients undergoing chronic hemodialysis were enrolled. The acute effect of a hemodialysis session on serum cytokine concentrations was assessed by comparison of pre-hemodialysis and post-hemodialysis determinations. Serum IL-1β, sIL-2R, IL-6, IL-8 and TNF-α levels were determined with chemiluminescence enzyme immunometric assays. A significant difference was not observed for IL-1β, IL-6, TNF-α, and sIL-2R concentrations in pre-hemodialysis and post-hemodialysis specimens (p＞0.05). Serum median (25th-75th percentiles) IL-8 concentration was 69.4 (34.9-110.3) pg/ml before hemodialysis, and decreased to 31.5 (18.0-78.8) pg/ml following hemodialysis (p: 0.006). Clearance of IL-8 increased by 0.47±0.08 pg/ml for each unit increase in pre-dialysis IL-8(p＜0.001) and decreased by 5.63±2.59 pg/ml for each unit increase in pre-dialysis urea mmol/l (p＜0.05). In conclusion, the results of our study demonstrate that a hemodialysis session markedly decreases IL-8 concentration, which is significantly affected by pre-dialysis concentrations, indicating that removal of IL-8 is a concentration gradient-dependent action, but does not change the serum levels of IL-1β, sIL-2R, IL-6, and TNF-α, underlining importance of the structural characteristics of the molecules.