Degradation of Metal−EDTA Complexes by Resting Cells of the Bacterial Strain DSM 9103

• Published in: Environmental science & technology Vol. 34; no. 9; pp. 1715 - 1720
• Main Authors: Satroutdinov, Aidar D, Dedyukhina, Emiliya G, Chistyakova, Tat‘yana I, Witschel, Margarete, Minkevich, Igor G, Eroshin, Valery K, Egli, Thomas
• Format: Journal Article
• Language: English
• Published: Easton American Chemical Society 01-05-2000
• Subjects: Agents; BACTERIA; Batch cell culture; Biochemistry; BIODEGRADATION; CADMIUM; Cells; Chelation; Chemicals; COBALT; COPPER; DSM 9103; EDTA; ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION; ENVIRONMENTAL SCIENCES; Ions; IRON; LEAD; metal-EDTA complexes; Metals; Microbiology; REMEDIAL ACTION; Toxicity; WASTE WATER; ZINC
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es981081q

Ethylenediaminetetraacetate (EDTA), an industrially important chelating agent, forms very stable complexes with di- and trivalent metal ions, and in both wastewater and natural waters it is normally present in the metal-associated form. Therefore, the influence of EDTA speciation on its utilization by the EDTA-degrading bacterial strain DSM 9103 was investigated. EDTA-grown cells harvested from the exponential phase of a batch culture were incubated with 1 mM of various EDTA species and the EDTA concentration in the assay was monitored as a function of time. Uncomplexed EDTA as well as complexes with low stability constants (MgEDTA2-, CaEDTA2-, and MnEDTA2-, stability constant < 1016) were found to be readily degraded to completion at a constant rate. For more stable EDTA chelates (i.e. chelates of Co2+, Cu2+, Zn2+, and Pb2+) the data suggest that these complexes were not used directly by the cells but that they had to dissociate prior to degradation. The rate of this dissociation step possibly determined the microbial degradation of these complexes. CdEDTA2- and Fe(III)EDTA- were not degraded within 48 h. In case of CdEDTA2- the toxicity of freed Cd2+ ions most likely prevented a significant degradation of the complex, whereas in case of Fe(III)EDTA- a combination of metal or complex toxicity and the very slow dissociation of the complex might explain the absence of degradation.