The RND efflux system ParXY affects siderophore secretion in Pseudomonas putida KT2440

• Published in: Microbiology spectrum Vol. 11; no. 6; p. e0230023
• Main Authors: Stein, Nicola Victoria, Eder, Michelle, Burr, Fabienne, Stoss, Sarah, Holzner, Lorenz, Kunz, Hans-Henning, Jung, Heinrich
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 12-12-2023
• Subjects: Environmental Microbiology; iron; ParXY; PP_3455; PP_3456; pyoverdine; Research Article; RND transporter; RND transporter; pyoverdine; MdtABC-OpmB; ParXY; PP_3455; PP_3456; iron; chloramphenicol; Pseudomonas putida KT2440; PvdRT-OpmQ; MexAB; siderophore
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.02300-23

Gram-negative bacteria from the
group are survivors in various environmental niches. For example, the bacteria secrete siderophores to capture ferric ions under deficiency conditions. Tripartite efflux systems are involved in the secretion of siderophores, which are also important for antibiotic resistance. For one of these efflux systems, the resistance-nodulation-cell division transporter ParXY from the model organism
KT2440, we show that it influences the secretion of the siderophore pyoverdine in addition to its already known involvement in antibiotic resistance. Phenotypically, its role in pyoverdine secretion is only apparent when other pyoverdine secretion systems are inactive. The results confirm that the different tripartite efflux systems have overlapping substrate specificities and can at least partially functionally substitute for each other, especially in important physiological activities such as supplying the cell with iron ions. This fact must be taken into account when developing specific inhibitors for tripartite efflux systems.