Glycoproteomic Profiling Provides Candidate Myocardial Infarction Predictors of Later Progression to Heart Failure

• Published in: ACS omega Vol. 4; no. 1; pp. 1272 - 1280
• Main Authors: Deleon-Pennell, Kristine Y, Ero, Osasere K, Ma, Yonggang, Padmanabhan Iyer, Rugmani, Flynn, Elizabeth R, Espinoza, Ingrid, Musani, Solomon K, Vasan, Ramachandran S, Hall, Michael E, Fox, Ervin R, Lindsey, Merry L
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 31-01-2019
• ISSN: 2470-1343; 2470-1343
• DOI: 10.1021/acsomega.8b02207

We hypothesized that identifying plasma glycoproteins that predict the development of heart failure following myocardial infarction (MI) could help to stratify subjects at risk. Plasma collected at visit 2 (2005–2008) from an MI subset of Jackson Heart Study participants underwent glycoproteomics and was grouped by the outcome: (1) heart failure hospitalization after visit 2 (n = 15) and (2) without hospitalization by 2012 (n = 45). Proteins were mapped for biological processes and functional pathways using Ingenuity Pathway Analysis and linked to clinical characteristics. A total of 198 glycopeptides corresponding to 88 proteins were identified (data available via ProteomeXchange with identifier PXD009870). Of these, 14 glycopeptides were significantly different between MI and MI + HF groups and corresponded to apolipoprotein (Apo) F, transthyretin, Apo C-IV, prostaglandin-D2 synthase, complement C9, and CD59 (p < 0.05 for all). All proteins were elevated in the MI + HF group, except CD59, which was lower. Four canonical pathways were upregulated in the MI + HF group (p < 0.05 for all): acute phase response, liver X receptor/retinoid X receptor, and macrophage reactive oxygen species generation. The coagulation pathway was significantly downregulated in the MI + HF group (p < 0.05). Even after adjustment for age and sex, Apo F was associated with the increased risk for heart failure (OR = 21.84; 95% CI 3.20–149.14). In conclusion, glycoproteomic profiling provided candidate early MI predictors of later progression to heart failure.