Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

• Published in: Chemical reviews Vol. 122; no. 10; pp. 10036 - 10086
• Main Authors: Ghassemi, Nader, Poulhazan, Alexandre, Deligey, Fabien, Mentink-Vigier, Frederic, Marcotte, Isabelle, Wang, Tuo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 25-05-2022
• Subjects: Algae; Antiinfectives and antibacterials; Bacteria; Biofilms; Biofuels; Biomaterials; Biomedical materials; Biomolecules; Carbohydrates; Cell Wall - chemistry; Fungi; Magnetic Resonance Spectroscopy; NMR; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; Plants; Solid state
• ISSN: 0009-2665; 1520-6890
• DOI: 10.1021/acs.chemrev.1c00669

Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for supporting cell integrity and function and regulating intercellular communication. These biomaterials are also of significant interest to the production of biofuels and the development of antimicrobial treatment. Solid-state nuclear magnetic resonance (ssNMR) and magic-angle spinning-dynamic nuclear polarization (MAS-DNP) are uniquely powerful for understanding the conformational structure, dynamical characteristics, and supramolecular assemblies of carbohydrates and other biomolecules in ECMs. This review highlights the recent high-resolution investigations of intact ECMs and native cells in many organisms spanning across plants, bacteria, fungi, and algae. We spotlight the structural principles identified in ECMs, discuss the current technical limitation and underexplored biochemical topics, and point out the promising opportunities enabled by the recent advances of the rapidly evolving ssNMR technology.