Genomic Analysis Reveals Antibiotic-Susceptible Clones and Emerging Resistance in Neisseria gonorrhoeae in Saskatchewan, Canada

• Published in: Antimicrobial agents and chemotherapy Vol. 64; no. 9
• Main Authors: Parmar, Nidhi R, Singh, Reema, Martin, Irene, Perera, Sumudu R, Demczuk, Walter, Kusalik, Anthony, Minion, Jessica, Dillon, Jo-Anne R
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 20-08-2020
• Subjects: Epidemiology and Surveillance; transmission linkages; Neisseria gonorrhoeae; single-nucleotide polymorphisms; antimicrobial resistance prediction; whole-genome sequencing; strain types
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/aac.02514-19

Whole-genome sequencing was used to identify mutations in antibiotic resistance-conferring genes to compare susceptibility predictions with MICs and to ascertain strain types in 99 isolates of
Genotypes associated with susceptibility, as well as MIC creep or emerging resistance, were noted. Phylogenomic analysis revealed three distinctive clades and putative gonococcal transmission linkages involving a tetracycline-resistant
outbreak and the clonal spread of susceptible isolates in men.