Aptamer-Based Surface-Enhanced Raman Scattering-Microfluidic Sensor for Sensitive and Selective Polychlorinated Biphenyls Detection

• Published in: Analytical chemistry (Washington) Vol. 87; no. 19; pp. 9555 - 9558
• Main Authors: Fu, Cuicui, Wang, Yi, Chen, Gang, Yang, Liyuan, Xu, Shuping, Xu, Weiqing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06-10-2015
• Subjects: Aluminum; Aluminum oxide; Aluminum Oxide - chemistry; Aptamers, Nucleotide - chemistry; Arrays; Channels; Chips; Metal Nanoparticles - chemistry; Metal oxides; Microfluidic Analytical Techniques - instrumentation; Microfluidics; Nanostructure; Nanotechnology; PCB; Peptides; Polychlorinated biphenyls; Polychlorinated Biphenyls - analysis; Scattering; Silicone resins; Silver; Silver - chemistry; Spectrum Analysis, Raman - instrumentation; Surface Properties
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.5b02508

A surface-enhanced Raman scattering (SERS) measurement of 3,3′,4,4′-tetrachlorobiphenyl (PCB77) with aptamer capturing in a microfluidic device was demonstrated. To construct the microfluidic chip, an ordered Ag nanocrown array was fabricated over a patterned polydimethylsiloxane (PDMS) that was achieved by replicating an anodic aluminum oxide (AAO) template. The patterned PDMS sheet was covered with another PDMS sheet having two input channel grooves to form a close chip. The Ag nanocrown array was used for the SERS enhancement area and the detection zone. PCB 77 aptamers were injected into one channel and the other allows for analytes (PCBs). The mercapto aptamers captured the targets in the mixed zone and were immobilized to the SERS detection zone via S–Ag bonds so as to further improve both the SERS sensitivity and selectivity of PCB77. Such an aptamer-based microfluidic chip realized a rapid SERS detection. The lowest detectable concentration of 1.0 × 10–8 M was achieved for PCB77. This work demonstrates that the aptamer-modified SERS microfluidic sensor can be utilized for selective detections of organic pollutants in the environment.