Localization and Quantification of Carbon-Centered Radicals on Any Amino Acid of a Protein

• Published in: Analytical chemistry (Washington) Vol. 79; no. 14; pp. 5444 - 5448
• Main Authors: Mousseau, G, Thomas, O. P, Oppilliart, S, Coirier, A, Salcedo-Serna, A, Thai, R, Beau, F, Renault, J.-P, Pin, S, Cintrat, J.-C, Rousseau, B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15-07-2007
• Subjects: Amino acids; Amino Acids - chemistry; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Animals; Biochemistry; Biochemistry, Molecular Biology; Biological and medical sciences; Carbon - analysis; Chemical Sciences; Chemistry; Exact sciences and technology; Free Radicals - analysis; Fundamental and applied biological sciences. Psychology; Life Sciences; Peptides; Peptides - chemistry; Proteins; Proteins - chemistry; Radiochemistry; Spectrometric and optical methods; Structural Biology; Tritium - analysis; Peptides; Hydrogen; Residue; Aminoacid; NMR spectrometry; Carbon; Radical; Protein; Quantitative analysis
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac070751k

A general strategy to localize and quantify carbon-centered radicals within proteins is described. The methodology was first exemplified on amino acids and then on a peptide. This method is applicable to any protein system regardless of size, and the site of hydrogen abstraction by •OH on all residues within proteins is easily and accurately detected.