Water-Soluble Carbohydrates and Fructan Structure Patterns from Agave and Dasylirion Species

• Published in: Journal of agricultural and food chemistry Vol. 54; no. 20; pp. 7832 - 7839
• Main Authors: Mancilla-Margalli, N.A, Lopez, M.G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 04-10-2006
• Subjects: Agave; Agave - chemistry; Biological and medical sciences; carbohydrate composition; Carbohydrate Conformation; carbohydrate content; Carbohydrates - chemistry; chemical structure; Chromatography, Thin Layer; Dasylirion; Environment; Food industries; fructans; Fructans - chemistry; Fundamental and applied biological sciences. Psychology; Glycosylation; Magnoliopsida - chemistry; Plant Stems - chemistry; Solubility; stems; Water; water-soluble carbohydrates; Water; Monocotyledones; fructans; partially methylated alditol acetates; Dasylirion; Acetate; branching; Agave; Gas chromatography; Alditol; Angiospermae; gas chromatography coupled to mass spectrometry; Agavaceae; Spermatophyta; Carbohydrate; Structure; Species; Mass spectrometry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf060354v

Fructans, storage carbohydrates with β-fructofuranosyl linkages, are found in ∼15% of higher plants. The metabolic flexibility of those molecules allows them easily to polymerize and depolymerize to soluble carbohydrates according to plant development stage and environmental conditions. In this work, water-soluble carbohydrates, including fructan structure patterns, were compared among Agave and Dasylirion species grown in different environmental regions in Mexico. Fructans were the main storage carbohydrate present in Agave stems, in addition to other carbohydrates related to its metabolism, whereas Dasylirion spp. presented a different carbohydrate distribution. A good correlation of water-soluble carbohydrate content with climatic conditions was observed. Fructans in Agave and Dasylirion genera were found in the form of polydisperse molecules, where structural heterogeneity in the same plant was evidenced by methylation linkage analysis and chromatographic methods. Fructans from the studied species were classified into three groups depending on DP and linkage-type abundance. These storage carbohydrates share structural characteristics with fructans in plants that belong to the Asparagales members. Agave and Dasylirion fructans can be categorized as graminans and branched neo-fructans, which we have termed agavins. Keywords: Agave; Dasylirion; fructans; branching; partially methylated alditol acetates; gas chromatography coupled to mass spectrometry