RNA-Binding Proteins PCBP1 and PCBP2 Are Critical Determinants of Murine Erythropoiesis

• Published in: Molecular and cellular biology Vol. 41; no. 9; p. e0066820
• Main Authors: Ji, Xinjun, Jha, Anupama, Humenik, Jesse, Ghanem, Louis R., Kromer, Andrew, Duncan-Lewis, Christopher, Traxler, Elizabeth, Weiss, Mitchell J., Barash, Yoseph, Liebhaber, Stephen A.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 24-08-2021; American Society for Microbiology
• Subjects: Aging - physiology; Animals; Cell Lineage; conditional gene inactivation; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryo, Mammalian - metabolism; Erythroid Cells - cytology; Erythropoiesis; Exons - genetics; Genetic Loci; Hematopoietic Stem Cells - metabolism; Mice; murine erythropoiesis; PCBP1; PCBP2; posttranscriptional controls; Research Article; RNA Splicing - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-binding proteins; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Spotlight; Transcriptome - genetics; conditional gene inactivation; RNA-binding proteins; murine erythropoiesis; PCBP1; posttranscriptional controls; PCBP2
• ISSN: 0270-7306; 1098-5549; 1098-5549
• DOI: 10.1128/MCB.00668-20

We previously demonstrated that the two paralogous RNA-binding proteins PCBP1 and PCBP2 are individually essential for mouse development: Pcbp1-null embryos are peri-implantation lethal, while Pcbp2-null embryos lose viability at midgestation. Midgestation Pcbp2
−/−
embryos revealed a complex phenotype that included loss of certain hematopoietic determinants. Whether PCBP2 directly contributes to erythropoietic differentiation and whether PCBP1 has a role in this process remained undetermined. Here, we selectively inactivated the genes encoding these two RNA-binding proteins during differentiation of the erythroid lineage in the developing mouse embryo. Individual inactivation of either locus failed to impact viability or blood formation. However, combined inactivation of the two loci resulted in midgestational repression of erythroid/hematopoietic gene expression, loss of blood formation, and fetal demise. Orthogonal ex vivo analyses of primary erythroid progenitors selectively depleted of these two RNA-binding proteins revealed that they mediate a combination of overlapping and isoform-specific impacts on hematopoietic lineage transcriptome, impacting both mRNA representation and exon splicing. These data lead us to conclude that PCBP1 and PCBP2 mediate functions critical to differentiation of the erythroid lineage.