Strategies for high cell density cultivation of Akkermansia muciniphila and its potential metabolism

• Published in: Microbiology spectrum Vol. 12; no. 1; p. e0238623
• Main Authors: Wu, Haiting, Qi, Shuhua, Yang, Ruixiong, Pan, Qihua, Lu, Yinghua, Yao, Chuanyi, He, Ning, Huang, Song, Ling, Xueping
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 11-01-2024
• Subjects: Akkermansia; Akkermansia muciniphila; Biomass; Carbon; cell surface hydrophobicity; Fermentation; Food Microbiology; high cell density; metabolites; out-membrane functional protein; Research Article; high cell density; out-membrane functional protein; Akkermansia muciniphila; cell surface hydrophobicity; metabolites
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.02386-23

Currently, there is significant interest in
as a promising next-generation probiotic, making it a hot topic in scientific research. However, to achieve efficient industrial production, there is an urgent need to develop an
culture method to achieve high biomass using low-cost carbon sources such as glucose. This study aims to explore the high-density fermentation strategy of
by optimizing the culture process. This study also employs techniques such as LC-MS and RNA-Seq to explain the possible regulatory mechanism of high-density cell growth and increased cell surface hydrophobicity facilitating cell colonization of the gut
culture. Overall, this research sheds light on the potential of
as a probiotic and provides valuable insights for future industrial production.