RBOH-Dependent ROS Synthesis and ROS Scavenging by Plant Specialized Metabolites To Modulate Plant Development and Stress Responses

• Published in: Chemical research in toxicology Vol. 32; no. 3; pp. 370 - 396
• Main Authors: Chapman, Jordan M, Muhlemann, Joëlle K, Gayomba, Sheena R, Muday, Gloria K
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 18-03-2019
• Subjects: Free Radical Scavengers - metabolism; NADPH Oxidases - metabolism; Plant Development; Plants - metabolism; Reactive Oxygen Species - metabolism; Signal Transduction; Stress, Physiological
• ISSN: 0893-228X; 1520-5010
• DOI: 10.1021/acs.chemrestox.9b00028

Reactive oxygen species (ROS) regulate plant growth and development. ROS are kept at low levels in cells to prevent oxidative damage, allowing them to be effective signaling molecules upon increased synthesis. In plants and animals, NADPH oxidase/respiratory burst oxidase homolog (RBOH) proteins provide localized ROS bursts to regulate growth, developmental processes, and stress responses. This review details ROS production via RBOH enzymes in the context of plant development and stress responses and defines the locations and tissues in which members of this family function in the model plant Arabidopsis thaliana. To ensure that these ROS signals do not reach damaging levels, plants use an array of antioxidant strategies. In addition to antioxidant machineries similar to those found in animals, plants also have a variety of specialized metabolites that scavenge ROS. These plant specialized metabolites exhibit immense structural diversity and have highly localized accumulation. This makes them important players in plant developmental processes and stress responses that use ROS-dependent signaling mechanisms. This review summarizes the unique properties of plant specialized metabolites, including carotenoids, ascorbate, tocochromanols (vitamin E), and flavonoids, in modulating ROS homeostasis. Flavonols, a subclass of flavonoids with potent antioxidant activity, are induced during stress and development, suggesting that they have a role in maintaining ROS homeostasis. Recent results using genetic approaches have shown how flavonols regulate development and stress responses through their action as antioxidants.