Direct Observation of the Release of Phenylalanine from Diphenylalanine Nanotubes

• Published in: Journal of the American Chemical Society Vol. 128; no. 21; pp. 6903 - 6908
• Main Authors: Sedman, Victoria L, Adler-Abramovich, Lihi, Allen, Stephanie, Gazit, Ehud, Tendler, Saul J. B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 31-05-2006
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Hot Temperature; Image Processing, Computer-Assisted; Microscopy, Atomic Force; Miscellaneous; Molecular biophysics; Nanotubes - chemistry; Phenylalanine - analogs & derivatives; Phenylalanine - chemistry; Physico-chemical properties of biomolecules; Spectrometry, Mass, Secondary Ion - methods; Atomic force microscopy; α-Aminoacid; Phenylalanine; Chemical stability; Nanotube; Secondary ion mass spectrometry; Surface topography; Thermal stability; Surface structure
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja060358g

The core recognition motif of the amyloidogenic β-amyloid polypeptide is a dipeptide of phenylalanine. This dipeptide readily self-assembles to form discrete, hollow nanotubes with high persistence lengths. The simplicity of the nanotube formation, combined with ideal physical properties, make these nanotubes highly desirable for a range of applications in bionanotechnology. To fully realize the potential of such structures, it is first necessary to gain a comprehensive understanding of their chemical and physical properties. Previously, the thermal stability of these nanotubes has been investigated by electron microscopy. Here, we further our understanding of the structural stability of the nanotubes upon dry-heating using the atomic force microscope (AFM), and for the first time identify their degradation product utilizing time-of-flight secondary-ion mass spectrometry. We show that the nanotubes are stable at temperatures up to 100 °C, but on heating to higher temperatures begin to lose their structural integrity with an apparent collapse in tubular structure. With further increases in temperature up to and above 150 °C, there is a degradation of the structure of the nanotubes through the release of phenylalanine building blocks. The breakdown of structure is observed in samples that are either imaged at elevated temperatures or imaged following cooling, suggesting that once phenylalanine is lost from the nanotubes they are susceptible to mechanical deformation by the imaging AFM probe. This temperature-induced plasticity may provide novel properties for these peptide nanotubes, including possible applications as scaffolds and drug delivery devices.