Comparison of Antimicrobial-Resistant Escherichia coli Isolates from Urban Raccoons and Domestic Dogs

• Published in: Applied and environmental microbiology Vol. 87; no. 15; p. e0048421
• Main Authors: Worsley-Tonks, Katherine E L, Gehrt, Stanley D, Miller, Elizabeth A, Singer, Randall S, Bender, Jeff B, Forester, James D, McKenzie, Shane C, Travis, Dominic A, Johnson, Timothy J, Craft, Meggan E
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 13-07-2021
• Subjects: Animal behavior; Animal health; Animals; Antimicrobial agents; Antimicrobial resistance; Bacteria; Cephalosporins; Chicago - epidemiology; Clusters; Dog Diseases - epidemiology; Dog Diseases - microbiology; Dogs; Dogs - microbiology; Domestic animals; Drug Resistance, Bacterial - genetics; E coli; Epidemiology; Escherichia coli; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Exposure; Female; Interfaces; Livestock; Male; Metropolitan areas; Nucleotides; Parks; Parks & recreation areas; Parks, Recreational; Phylogenetics; Phylogeny; Polymorphism; Polymorphism, Single Nucleotide; Procyonidae; Public and Environmental Health Microbiology; Raccoons - microbiology; Single-nucleotide polymorphism; Spatial analysis; Species; Suburban areas; Urban areas; Wild animals; Wildlife; Chicago; United States > US; Chicago Illinois; raccoon; phylogenetic; urban; Escherichia coli; interface; cephalosporin; dog
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00484-21

Wildlife can be exposed to antimicrobial-resistant bacteria (ARB) via multiple pathways. Spatial overlap with domestic animals is a prominent exposure pathway. However, most studies of wildlife-domestic animal interfaces have focused on livestock and little is known about the wildlife-companion animal interface. Here, we investigated the prevalence and phylogenetic relatedness of extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli from raccoons (Procyon lotor) and domestic dogs (Canis lupus familiaris) in the metropolitan area of Chicago, IL, USA. To assess the potential importance of spatial overlap with dogs, we explored whether raccoons sampled at public parks (i.e., parks where people and dogs could enter) differed in prevalence and phylogenetic relatedness of ESC-R E. coli to raccoons sampled at private parks (i.e., parks where people and dogs could not enter). Raccoons had a significantly higher prevalence of ESC-R E. coli (56.9%) than dogs (16.5%). However, the richness of ESC-R E. coli did not vary by host species. Further, core single-nucleotide polymorphism (SNP)-based phylogenetic analyses revealed that isolates did not cluster by host species, and in some cases displayed a high degree of similarity (i.e., differed by less than 20 core SNPs). Spatial overlap analyses revealed that ESC-R E. coli were more likely to be isolated from raccoons at public parks than raccoons at private parks, but only for parks located in suburban areas of Chicago, not urban areas. That said, ESC-R E. coli isolated from raccoons did not genetically cluster by park of origin. Our findings suggest that domestic dogs and urban/suburban raccoons can have a diverse range of ARB, some of which display a high degree of genetic relatedness (i.e., differ by less than 20 core SNPs). Given the differences in prevalence, domestic dogs are unlikely to be an important source of exposure for mesocarnivores in urbanized areas.
Antimicrobial-resistant bacteria (ARB) have been detected in numerous wildlife species across the globe, which may have important implications for human and animal health. Wildlife can be exposed to ARB via numerous pathways, including via spatial overlap with domestic animals. However, the interface with domestic animals has mostly been explored for livestock and little is known about the interface between wild animals and companion animals. Our work suggests that urban and suburban wildlife can have similar ARB to local domestic dogs, but local dogs are unlikely to be a direct source of exposure for urban-adapted wildlife. This finding is important because it underscores the need to incorporate wildlife into antimicrobial resistance surveillance efforts, and to investigate whether certain urban wildlife species could act as additional epidemiological pathways of exposure for companion animals, and indirectly for humans.