A Simple and Practical Spreadsheet-Based Method to Extract Single-Molecule Dissociation Kinetics from Variable Loading-Rate Force Spectroscopy Data
A simple, practical, and model-free method is presented, by which kinetic data can be extracted from variable loading-rate single molecule dynamic force spectroscopy experiments. The constant sampling rate of digital acquisition facilitates the sorting of multiple force curves into histograms that r...
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| Published in: | Journal of physical chemistry. C Vol. 112; no. 49; pp. 19163 - 19167 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Chemical Society
11-12-2008
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| Online Access: | Get full text |
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| Summary: | A simple, practical, and model-free method is presented, by which kinetic data can be extracted from variable loading-rate single molecule dynamic force spectroscopy experiments. The constant sampling rate of digital acquisition facilitates the sorting of multiple force curves into histograms that reflect the collective force−time history of the bonds of interest. Combining the force−time history with similar histograms of force-induced events, in particular bond rupture, gives a direct measure of rate vs applied force. The utility of the method is demonstrated through its application to simulated data. |
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| Bibliography: | istex:63E71FA68DB41E9525107938632865A7CC82895F Simulation method, representative time and force histograms, as well as the kinetics fits summarized in Table are provided. This material is available free of charge via the Internet at http://pubs.acs.org. ark:/67375/TPS-BJ79KTQP-R ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1932-7447 1932-7455 |
| DOI: | 10.1021/jp806649a |