Phosphatidyl-Tris rather than N-acylphosphatidylserine is synthesized by Rhodopseudomonas sphaeroides grown in Tris-containing media

• Published in: Biochemistry (Easton) Vol. 30; no. 7; pp. 1746 - 1751
• Main Authors: Schmid, Patricia C, Kumar, V. V, Weis, Barbara K, Schmid, Harald H. O
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 19-02-1991
• Subjects: 1,2-dioleoyl-sn-glycero-3-phospho-Tris; 550601 - Medicine- Unsealed Radionuclides in Diagnostics; Acylation; AMINO ACIDS; BACTERIA; Bacteriology; BARYONS; BIOCHEMISTRY; Biological and medical sciences; BIOSYNTHESIS; CARBON 13; CARBON ISOTOPES; CARBOXYLIC ACIDS; CHEMISTRY; Culture Media; ELEMENTARY PARTICLES; ESTERS; EVEN-ODD NUCLEI; FERMIONS; Fundamental and applied biological sciences. Psychology; Glycerophospholipids; HADRONS; HYDROXY ACIDS; INFRARED SPECTRA; ISOTOPES; LIGHT NUCLEI; LIPIDS; MAGNETIC RESONANCE; Magnetic Resonance Spectroscopy - methods; Metabolism. Enzymes; Methylation; Microbiology; MICROORGANISMS; NUCLEAR MAGNETIC RESONANCE; NUCLEI; NUCLEONS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; Phosphatidic Acids - metabolism; Phosphatidylserines - metabolism; PHOSPHOLIPIDS; PROTONS; RADIOLOGY AND NUCLEAR MEDICINE; RESONANCE; Rhodobacter sphaeroides - metabolism; RHODOPSEUDOMONAS; SERINE; SPECTRA; STABLE ISOTOPES; SYNTHESIS; Tromethamine - metabolism; Infrared spectrometry; Substrate; Thin layer chromatography; Rhodospirillales; Aminoalcohol; Rhodobacter sphaeroides; Phospholipid; Bacteria; Rhodospirillaceae; Biosynthesis; NMR spectrometry; Triol
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00221a003

We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these data with those reported for a phospholipid isolated from Rhodopseudomonas sphaeroides grown in Tris-supplemented media [Donohue et al. (1982) Biochemistry 21, 2765-2773] indicates that R. sphaeroides synthesizes phosphatidyl-Tris rather than N-acyl-PS.