Electrochemical Nanoneedle Biosensor Based on Multiwall Carbon Nanotube

• Published in: Analytical chemistry (Washington) Vol. 78; no. 2; pp. 617 - 620
• Main Authors: Boo, Hankil, Jeong, Ran-A, Park, Sejin, Kim, Keun Soo, An, Kay Hyeok, Lee, Young Hee, Han, Ji Hyung, Kim, Hee Chan, Chung, Taek Dong
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15-01-2006
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbon; Chemistry; Dopamine - analysis; Electrochemical methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Glutamic Acid - analysis; Methods. Procedures. Technologies; Microscopy, Electron, Scanning; Nanotubes; Nanotubes, Carbon - chemistry; Potentiometry; Various methods and equipments; Dopamine; Enzyme; Electrochemical method; Biosensor; Tungsten; Differential impulse voltamperometry; Amperometry; Carbon nanotubes; Chemical sensor; Glutamate; Analytical function
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac0508595

We report the fabrication and analytical functions of a biosensor based on a nanoneedle consisting of a multiwall carbon nanotube attached to the end of an etched tungsten tip. The devised electrode is the smallest needle-type biosensor reported to date. The nanoneedles prepared in this work are 30 nm in diameter and 2−3 μm in length. Dopamine and glutamate, which are physiologically important neurotransmitters, were successfully detected using these nanoneedles. Bare nanoneedles detected dopamine in the range from 100 to 1000 μM by differential pulse voltammetry, and enzyme-modified nanoneedles were able to respond to glutamate in the 100−500 μM range by potentiostatic amperometry.