Immunomodulatory and Antileishmanial Activity of Phenylpropanoid Dimers Isolated from Nectandra leucantha

• Published in: Journal of natural products (Washington, D.C.) Vol. 78; no. 4; pp. 653 - 657
• Main Authors: Costa-Silva, Thais Alves da, Grecco, Simone S, de Sousa, Fernanda S, Lago, João Henrique G, Martins, Euder G. A, Terrazas, César A, Varikuti, Sanjay, Owens, Katherine L, Beverley, Stephen M, Satoskar, Abhay R, Tempone, Andre G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society and American Society of Pharmacognosy 24-04-2015
• Subjects: Animals; Anisoles - chemistry; Anisoles - isolation & purification; Anisoles - pharmacology; Antiprotozoal Agents - chemistry; Antiprotozoal Agents - isolation & purification; Antiprotozoal Agents - pharmacology; Brazil; Immunologic Factors - chemistry; Immunologic Factors - isolation & purification; Immunologic Factors - pharmacology; Inhibitory Concentration 50; Interleukin-10; Interleukin-6; Lauraceae - chemistry; Leishmania donovani; Leishmania donovani - drug effects; Leishmaniasis - drug therapy; Macrophages, Peritoneal - drug effects; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Structure; Nectandra; Nitric Oxide - metabolism; Phenylpropionates - chemistry; Phenylpropionates - isolation & purification; Phenylpropionates - pharmacology; Plant Stems - chemistry; Brazil
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np500809a

Three phenylpropanoid dimers (1–3) including two new metabolites were isolated from the extract of the twigs of Nectandra leucantha using antileishmanial bioassay-guided fractionation. The in vitro antiparasitic activity of the isolated compounds against Leishmania donovani parasites and mammalian cytotoxicity and immunomodulatory effects were evaluated. Compounds 1–3 were effective against the intracellular amastigotes within macrophages, with IC50 values of 26.7, 17.8, and 101.9 μM, respectively. The mammalian cytotoxicity, given by the 50% cytotoxic concentration (CC50), was evaluated against peritoneal macrophages. Compounds 1 and 3 were not toxic up to 290 μM, whereas compound 2 demonstrated a CC50 value of 111.2 μM. Compounds 1–3 also suppressed production of disease exacerbatory cytokines IL-6 and IL-10 but had minimal effect on nitric oxide production in L. donovani-infected macrophages, indicating that antileishmanial activity of these compounds is mediated via an NO-independent mechanism. Therefore, these new natural products could represent promising scaffolds for drug design studies for leishmaniasis.