Size-, Composition- and Shape-Dependent Toxicological Impact of Metal Oxide Nanoparticles and Carbon Nanotubes toward Bacteria

• Published in: Environmental science & technology Vol. 43; no. 21; pp. 8423 - 8429
• Main Authors: Simon-Deckers, Angélique, Loo, Sylvain, Mayne-L’hermite, Martine, Herlin-Boime, Nathalie, Menguy, Nicolas, Reynaud, Cécile, Gouget, Barbara, Carrière, Marie
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-11-2009
• Subjects: Applied sciences; Bacteria; Bacteria - cytology; Bacteria - drug effects; Bacteria - ultrastructure; Carbon; Chemical Sciences; Crystals; Culture Media; E coli; Ecotoxicology and Human Environmental Health; Effects; Environmental Exposure - analysis; Escherichia coli; Exact sciences and technology; Intracellular Space - drug effects; Intracellular Space - metabolism; Metal Nanoparticles - chemistry; Metal Nanoparticles - microbiology; Metal Nanoparticles - toxicity; Metal Nanoparticles - ultrastructure; Metal oxides; Microbial Viability - drug effects; Nanoparticles; Nanotubes; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - microbiology; Nanotubes, Carbon - toxicity; or physical chemistry; Oxides - chemistry; Oxides - toxicity; Particle Size; Pollution; Reactive Oxygen Species - metabolism; Studies; Theoretical and; Toxicity; Surface charge; Nanoparticle; Toxicity; Escherichia coli; Optimization; Transport process; Health and environment; Ecosystem; Morphology; Bacteria; Chemical composition; Public health; Enterobacteriaceae
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es9016975

Ecotoxicological effects of nanoparticles (NP) are still poorly documented while their commercialization for industrial and household applications increases. The aim of this study was to evaluate the influence of physicochemical characteristics on metal oxide NP and carbon nanotubes toxicological effects toward bacteria. Two strains of bacteria, Cupriavidus metallidurans CH34 and Escherichia coli MG1655 were exposed to TiO2 or Al2O3 NP or to multiwalled-carbon nanotubes (MWCNT). Particular attention was paid on optimizing NP dispersion to obtain nonagglomerated suspensions. Our results show that NP toxicity depends on their chemical composition, size, surface charge, and shape but not on their crystalline phase. MWCNT toxicity does not depend on their purity. Toxicity also depends on the bacterial strain: E. coli MG1655 is sensitive to NP, whereas C. metallidurans CH34 is not. Interestingly, NP are accumulated in both bacterial strains, and association between NP and bacteria is necessary for bacterial death to occur. NP may then represent a danger for the environment, causing the disappearance of some sensitive bacterial strains such as E. coli MG1655, but also being mobilized by nonsensitive strains such as C. metallidurans CH34 and transported through the whole ecosystem.