Cone degeneration in aging and age-related macular degeneration

• Published in: Archives of ophthalmology (1960) Vol. 127; no. 4; p. 483
• Main Authors: Shelley, Elizabeth J, Madigan, Michele C, Natoli, Riccardo, Penfold, Philip L, Provis, Jan M
• Format: Journal Article
• Language: English
• Published: United States 01-04-2009
• Subjects: Adult; Aged; Aged, 80 and over; Aging; Animals; Axons - metabolism; Biomarkers - metabolism; Female; Fluorescent Antibody Technique, Indirect; Humans; Macular Degeneration - metabolism; Macular Degeneration - pathology; Male; Middle Aged; Opsins - metabolism; Rats; Rats, Sprague-Dawley; Retinal Cone Photoreceptor Cells - metabolism; Retinal Cone Photoreceptor Cells - pathology; Rhodopsin - metabolism; Tissue Donors; Young Adult
• ISSN: 1538-3601
• DOI: 10.1001/archophthalmol.2008.622

To examine the morphological features of macular photoreceptors in histologically normal retina from normal donor eyes and eyes with age-related macular degeneration (AMD).
The macular region was excised from 18 donor eyes (aged 22-96 years) and cryosectioned. Sections were stained with hematoxylin-eosin or double immunolabeled using opsin antibodies or synaptic markers.
Three of 8 retinas studied in detail had AMD lesions; the remainder were histologically normal. Immunoreactivity to cone opsin was abnormal in parts of all retinas (3.5%-95.0% of each sample) and was associated with swelling of and altered immunoreactivity in the cone distal axon. In non-AMD retinas, the anomalies were mainly in nonfoveal macular locations. The nature of the anomalies was identical in non-AMD retinas and in parts of AMD retinas adjacent to overt degeneration.
Redistribution of opsin and anomalies in the distal cone axon are common in the aging human macula and may indicate susceptibility to AMD.
The findings are consistent with tests of cone function in aging and early AMD, which suggests that integrated cone functions--including contrast sensitivity, color matching, and short wavelength-sensitive cone sensitivity--are the most reliable prognostic indicators of progression in AMD.