Qualitative and Quantitative Sugar Profiling in Olive Fruits, Leaves, and Stems by Gas Chromatography−Tandem Mass Spectrometry (GC-MS/MS) after Ultrasound-Assisted Leaching

• Published in: Journal of agricultural and food chemistry Vol. 58; no. 23; pp. 12292 - 12299
• Main Authors: Gómez-González, Soledad, Ruiz-Jiménez, José, Priego-Capote, Feliciano, Luque de Castro, Marı́a Dolores
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 08-12-2010
• Subjects: Biological and medical sciences; Carbohydrates - chemistry; Carbohydrates - isolation & purification; Chemical Composition of Foods/Feeds; cultivars; food analysis; food composition; Food engineering; Food industries; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; gas chromatography; Gas Chromatography-Mass Spectrometry - methods; General aspects; leaching; leaves; mass spectrometry; Olea - chemistry; Olea europaea; olives; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Leaves - chemistry; Plant Stems - chemistry; qualitative analysis; quantitative analysis; stems; sugar content; ultrasonic treatment; Ultrasonics; Carbohydrates; ultrasound-assisted extraction; Olea europaea; GC-MS/MS; Oleaceae; Fruit; Plant leaf; Leaching; Stem; Olive; Gas chromatography; Plant part; Dicotyledones; Mass spectrometry MS/MS; Angiospermae; Spermatophyta; Extraction; Carbohydrate; Ultrasound; Sugar
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf102350s

Qualitative and quantitative profiling of sugars in vegetal materials from Olea europaea cultivars is here reported. Vegetal tissues from olive fruits, leaves, and stems have been characterized by determination of 22 compounds belonging to monosaccharides, disaccharides, trisaccharides, sugar carboxylic acids and alcohols, cyclic polyols, and derived compounds. Sugar isolation was carried out by leaching into a 2:1 dichloromethane/methanol extraction solution under ultrasonic assistance. Multivariate optimization made possible complete isolation of the target fraction in 10 min with an efficiency similar to that provided by a conventional protocol based on 24 h maceration of the vegetal samples. An aliquot of the extract was dried and reconstituted for silylation prior to GC-MS/MS analysis for selective and sensitive identification/quantitation of sugars. Monitoring the target product ions generated after isolation of the precursor ions for each analyte increases the selectivity of the method. The proposed approach is of particular interest for characterization of the sugar fraction in O. europaea, which is of great relevance because of the role of sugars in the metabolism of lipids, proteins, and antioxidants.