Production of Odd-Carbon Dicarboxylic Acids in Escherichia coli Using an Engineered Biotin–Fatty Acid Biosynthetic Pathway

• Published in: Journal of the American Chemical Society Vol. 139; no. 13; pp. 4615 - 4618
• Main Authors: Haushalter, Robert W, Phelan, Ryan M, Hoh, Kristina M, Su, Cindy, Wang, George, Baidoo, Edward E. K, Keasling, Jay D
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05-04-2017; American Chemical Society (ACS)
• Subjects: INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/jacs.6b11895

Dicarboxylic acids are commodity chemicals used in the production of plastics, polyesters, nylons, fragrances, and medications. Bio-based routes to dicarboxylic acids are gaining attention due to environmental concerns about petroleum-based production of these compounds. Some industrial applications require dicarboxylic acids with specific carbon chain lengths, including odd-carbon species. Biosynthetic pathways involving cytochrome P450-catalyzed oxidation of fatty acids in yeast and bacteria have been reported, but these systems produce almost exclusively even-carbon species. Here we report a novel pathway to odd-carbon dicarboxylic acids directly from glucose in Escherichia coli by employing an engineered pathway combining enzymes from biotin and fatty acid synthesis. Optimization of the pathway will lead to industrial strains for the production of valuable odd-carbon diacids.