Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV‑2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites

• Published in: ACS applied bio materials Vol. 5; no. 5; pp. 2421 - 2430
• Main Authors: Sadique, Mohd. Abubakar, Yadav, Shalu, Ranjan, Pushpesh, Khan, Raju, Khan, Firoz, Kumar, Ashok, Biswas, Debasis
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16-05-2022
• Subjects: Antibodies; Biosensing Techniques - methods; COVID-19 - diagnosis; Gold - chemistry; Graphite; Humans; Immunoassay - methods; Metal Nanoparticles - chemistry; Nanocomposites - chemistry; SARS-CoV-2; electrochemical immunosensor; SARS-CoV-2 antigen; SARS-CoV-2 antibody; electrodeposition; GO−Au nanocomposites
• ISSN: 2576-6422; 2576-6422
• DOI: 10.1021/acsabm.2c00301

In this work, we report a facile synthesis of graphene oxide–gold (GO–Au) nanocomposites by electrodeposition. The fabricated electrochemical immunosensors are utilized for the dual detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen and SARS-CoV-2 antibody. The GO–Au nanocomposites has been characterized by UV–vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) for its biosensing properties. The linear detection range of the SARS-CoV-2 antigen immunosensor is 10.0 ag mL–1 to 50.0 ng mL–1, whereas that for the antibody immunosensor ranges from 1.0 fg mL–1 to 1.0 ng mL–1. The calculated limit of detection (LOD) of the SARS-CoV-2 antigen immunosensor is 3.99 ag mL–1, and that for SARS-CoV-2 antibody immunosensor is 1.0 fg mL–1 with high sensitivity. The validation of the immunosensor has also been carried out on patient serum and patient swab samples from COVID-19 patients. The results suggest successful utilization of the immunosensors with a very low detection limit enabling its use in clinical samples. Further work is needed for the standardization of the results and translation in screen-printed electrodes for use in portable commercial applications.